亚洲精品一线,日本精品韩,五月天婷婷色悠悠激情,在线观看日本高清不卡三区四区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè)  >  技術(shù)文章  >  TOV-21G 人卵巢癌細(xì)胞

TOV-21G 人卵巢癌細(xì)胞

更新時(shí)間:2016-08-01      點(diǎn)擊次數(shù):2954
Designations:TOV-21G
Depositors: University of Montreal
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Homo sapiens deposited as human
Morphology:epithelial
 
Source:Organ: ovary 
Tumor Stage: grade 3, stage III 
Disease: primary malignant adenocarcinoma; clear cell carcinoma
Cellular Products:keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
Tumorigenic:Yes
Oncogene:p53 + (wild type)
DNA Profile (STR):Amelogenin: X 
CSF1PO: 13,15 
D13S317: 11,12 
D16S539: 10,12 
D5S818: 12,13 
D7S820: 12 
THO1: 7,9.3 
TPOX: 8,11 
vWA: 17
Cytogenetic Analysis:47, XX, +10 [49408 ]
Age:62 years
Gender:female
Comments:This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer. 
Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
    Temperature: 37.0°C
Subculturing:Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37?C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended 
Medium Renewal: Every 3 to 4 days
Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
References:

42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

CoC1人卵巢癌細(xì)胞系Lec1倉(cāng)鼠卵巢細(xì)胞
A2780人卵巢癌細(xì)胞OVAC卵巢癌細(xì)胞
Anglne人卵巢癌細(xì)胞系NIH:OVCAR-3人卵巢癌細(xì)胞
Caov-3人乳突狀卵巢腺癌細(xì)胞PA-1人卵巢畸胎瘤細(xì)胞
CHO中國(guó)倉(cāng)鼠卵巢細(xì)胞Pr-HNV8菜青蟲卵巢細(xì)胞
CHO/dhFr-倉(cāng)鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷RP70-a-CHO-0.8MTX中國(guó)倉(cāng)鼠卵巢細(xì)胞
CHO-K1中國(guó)倉(cāng)鼠卵巢細(xì)胞SF9晚秋粒蟲卵巢細(xì)胞
CHO-pt轉(zhuǎn)血小板基因倉(cāng)鼠卵巢細(xì)胞SKOV3人卵巢癌細(xì)胞
CHO-S中國(guó)倉(cāng)鼠卵巢懸浮細(xì)胞SKOV-3/DDP卵巢癌順鉑耐藥株
CoC1人卵巢癌細(xì)胞系SW1910人卵巢癌細(xì)胞
CoC2 卵巢癌 SW626人卵巢腺癌細(xì)胞
CTLA4 Ig-24中國(guó)倉(cāng)鼠卵巢細(xì)胞TOV-21G人卵巢癌
ES-2人卵巢透明細(xì)胞癌ZA1轉(zhuǎn)S9基因倉(cāng)鼠卵巢細(xì)胞
HO-8910人卵巢癌細(xì)胞ZA6轉(zhuǎn)S9基因倉(cāng)鼠卵巢細(xì)胞
HO-8910PM人高轉(zhuǎn)移卵巢癌細(xì)胞 Cho-K1倉(cāng)鼠卵巢細(xì)胞亞株
HOC1卵巢癌細(xì)胞SK-OV-3人卵巢癌細(xì)胞
Lec1倉(cāng)鼠卵巢細(xì)胞Sf9昆蟲卵巢細(xì)胞
CHO-K1倉(cāng)鼠卵巢細(xì)胞亞株ES-2人卵巢透明細(xì)胞癌
CHO/dhFr-倉(cāng)鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷SK-OV-3人卵巢癌細(xì)胞TOV-21G 人卵巢癌細(xì)胞TOV-21G 人卵巢
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
麻豆传媒在线观看小视频 | 麻豆一区 二区 三区 在线观看 | 女同精品久久| 国内外成人视频你懂的| 欧美日韩人妻偷拍| 亚洲福利精品网址| 98中文字幕中文字幕| 射精专区一区| 欧美高清H一区| 久久亚洲精品少妇综合| 天天透天天爽天天爱| AV免费阅在线免费观看| 你懂的91视频国产| 东京热免费成人电影| 亚洲熟妇se| 国产原创久久麻豆亚洲国产欧美 | 久久久成人剧场文化传媒| 婷婷五月天中文字幕一区二区 | 午夜欧美日韩成人无码观看免费| 伦理片一区二区在线观看| 射射亚洲精品| 久久精品亚洲无码新一区2区| 美曰韩一区| 欧美精品成人一级黄片| 人妻av网| 啪啪啪啪啪啪啪啪亚洲一区二区| 久热这里只有精品欧美| 2019亚洲午夜影院| 日韩精品午夜激情| 大陆精品一区| 91精品欧美激情| 亚洲一区二区三视频图片| 麻豆传媒出品在线| 操逼柴丹日本| 久久久久久久久久久国产毛片| 麻豆网站啊啊啊| 婷婷爱网成人| 日韩欧美中文字幕免费| 日本成人99| 日本 啪啪视频| 日本女人的午夜屁股|